Webinar Summary: Introduction to Flow Cytometry

November 7, 2011; 12:30 pm–2:00 pm
Ligand Binding Assay Bioanalytical focus group
Flow Cytometry Action program committee
Conducted by Nick Jones, Lynette Brown, MS, CHT, Wendy White, Ph.D., and Christopher Wiwi, Ph.D.
Moderated by Virginia Litwin, Ph.D., Covance
Supported by a grant from Sanofi

SanofiAventis logo



About the Webinar

Flow cytometry is a very powerful method of analyzing cells in which cell properties are detected as cells flow in a narrow stream through a measuring device (i.e., flow cytometer). Flow cytometry is a technology with many applications. While flow cytometry has been around for many years, constant development and research have opened more applications and flexibility to the platform. This webinar will provide an overview of key concepts of flow cytometry and its various uses. The focus will be on understanding the basic concepts and utility of this technology.

Webinar Agenda 

  • What is flow cytometry?
  • What are the applications in drug development?
  • Instrumentation
  • Types of flow cytometric analysis
  • Fluorescence reagent
  • Quantitation/Calibration

About the Presenters 

brownLynette Brown, M.S., CHT, is the president and cofounder of Flow Contract Site Laboratory LLC, which specializes in services focused on flow cytometry for all phases of drug development. She received her master’s degree in clinical leadership with an emphasis in clinical research administration from George Washington University and her bachelor's degree in zoology from the University of Washington. Lynette has over 10+ years in flow cytometry starting with performing flow cytometry cross-matching for organ donation at the Puget Sound Blood Center, Seattle, Wash. During her time at the Blood Center, she also earned her certification as a Certified Histocompatibility Technologist.

jonesNick Jones, B.S., is the manager of Assay Development and Validation–Flow Cytometry for Esoterix Clinical Trials Services, a Division of LabCorp, based in Brentwood, Tenn. Active in the field of flow cytometry assay development since 2000, Nicholas leads the group responsible for assay development, validation, and implementation into the family of Esoterix Clinical Trials Services laboratories within the United States, Europe, and Asia for global clinical trials flow cytometry support. Additionally, he oversees the implementation and standardization of instrumentation, reagents and flow cytometry processes across Esoterix Clinical Trials Services locations to ensure seamless data consistency across multiple locations.

whiteWendy White, Ph.D., is a group and subteam leader at MedImmune LLC providing strategic and tactical support to antibody-based drug candidates during late stage preclinical and clinical development. She leads a group of scientists in the development and subsequent execution of analytical approaches to biomarker analyses using a number of different technological approaches.White received her master's degree and doctorate degrees from Georgetown University in biology and her bachelor's degree in zoology from the University of New Hampshire.

cris wiwiChristopher Wiwi, Ph.D., is a senior scientist in the Cellular Process Development group at Celgene Cellular Therapeutics located in Warren, N.J. Chris leads the analytical development efforts for the advancement of Celgene Cellular Therapeutics’ novel cellular therapy candidates from research to preclinical, clinical, and commercial manufacturing. His team is responsible for the selection, development, and validation of bioanalytical methods to assess cellular product identity, purity, safety, quality, and potency. Chris received his bachelor's in biology from the University of Notre Dame and doctorate in cell and molecular biology from Boston University.